perkin elmer series 2000 instruments Search Results


li cor odyssey clx  (LI-COR)
98
LI-COR li cor odyssey clx
Li Cor Odyssey Clx, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/li cor odyssey clx/product/LI-COR
Average 98 stars, based on 1 article reviews
li cor odyssey clx - by Bioz Stars, 2026-03
98/100 stars
  Buy from Supplier
secondary antibodies conjugated hrp  (Jackson Immuno)
90
Jackson Immuno secondary antibodies conjugated hrp
Secondary Antibodies Conjugated Hrp, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/secondary antibodies conjugated hrp/product/Jackson Immuno
Average 90 stars, based on 1 article reviews
secondary antibodies conjugated hrp - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
lipofectamine rnaimax  (Thermo Fisher)
90
Thermo Fisher lipofectamine rnaimax
Lipofectamine Rnaimax, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lipofectamine rnaimax/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
lipofectamine rnaimax - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
ki 67 antibody  (Thermo Fisher)
86
Thermo Fisher ki 67 antibody
SORLA expression correlates with HER2 and promotes tumorigenesis in bladder carcinoma. ( a ) Immunohistological staining of TMA material and correlation analyses of SORLA and HER2 in clinical bladder cancer specimens. Numbers indicate staining intensity, 0=negative, 1=weak, 2=moderate, 3=high. ( b ) Western blot analysis of SORLA levels in scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 bladder cancer cells. GAPDH is a loading control. ( c ) Proliferation of scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 cells. Proliferation data are mean ± s.d. (n = 4 independent experiments; statistical analysis: two-way ANOVA). ( d ) Analysis of tumor growth of subcutaneously injected 5637 cells, with transient SORLA (siSORLA #3) or scramble (siCTRL) silencing, at day 29 in nude mice. Data are displayed as box plots (n = 9-10 mice/group; statistical analysis: unpaired Student’s t -test). ( e ) <t>Ki-67</t> and TUNEL staining of tumor samples prepared as described in (d) and quantifications displayed as box plots (n = 9-10 mice/group; statistical analysis: Mann-Whitney test). ( f ) Schematic illustrating the role of SORLA in regulating the oncogenic fitness of HER2-driven cancer cells. SORLA associates with HER2 via its extracellular domain, co-traffics with HER2 and facilitates HER2 recycling to the plasma membrane to support HER2 downstream signaling. In the absence of SORLA, HER2 becomes localized to enlarged, partially dysfunctional lysosomes resulting in defective HER2 signaling and increased sensitivity to CADs such as ebastine. End: endosome, Lys: lysosome. Box plots represent median and 25 th and 75 th percentiles (interquartile range; IQR) and whiskers extend to maximum and minimum values.
Ki 67 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ki 67 antibody/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
ki 67 antibody - by Bioz Stars, 2026-03
86/100 stars
  Buy from Supplier
cellometer auto 2000  (Nexcelom Bioscience)
90
Nexcelom Bioscience cellometer auto 2000
SORLA expression correlates with HER2 and promotes tumorigenesis in bladder carcinoma. ( a ) Immunohistological staining of TMA material and correlation analyses of SORLA and HER2 in clinical bladder cancer specimens. Numbers indicate staining intensity, 0=negative, 1=weak, 2=moderate, 3=high. ( b ) Western blot analysis of SORLA levels in scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 bladder cancer cells. GAPDH is a loading control. ( c ) Proliferation of scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 cells. Proliferation data are mean ± s.d. (n = 4 independent experiments; statistical analysis: two-way ANOVA). ( d ) Analysis of tumor growth of subcutaneously injected 5637 cells, with transient SORLA (siSORLA #3) or scramble (siCTRL) silencing, at day 29 in nude mice. Data are displayed as box plots (n = 9-10 mice/group; statistical analysis: unpaired Student’s t -test). ( e ) <t>Ki-67</t> and TUNEL staining of tumor samples prepared as described in (d) and quantifications displayed as box plots (n = 9-10 mice/group; statistical analysis: Mann-Whitney test). ( f ) Schematic illustrating the role of SORLA in regulating the oncogenic fitness of HER2-driven cancer cells. SORLA associates with HER2 via its extracellular domain, co-traffics with HER2 and facilitates HER2 recycling to the plasma membrane to support HER2 downstream signaling. In the absence of SORLA, HER2 becomes localized to enlarged, partially dysfunctional lysosomes resulting in defective HER2 signaling and increased sensitivity to CADs such as ebastine. End: endosome, Lys: lysosome. Box plots represent median and 25 th and 75 th percentiles (interquartile range; IQR) and whiskers extend to maximum and minimum values.
Cellometer Auto 2000, supplied by Nexcelom Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cellometer auto 2000/product/Nexcelom Bioscience
Average 90 stars, based on 1 article reviews
cellometer auto 2000 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
primary antibodies  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc primary antibodies
SORLA expression correlates with HER2 and promotes tumorigenesis in bladder carcinoma. ( a ) Immunohistological staining of TMA material and correlation analyses of SORLA and HER2 in clinical bladder cancer specimens. Numbers indicate staining intensity, 0=negative, 1=weak, 2=moderate, 3=high. ( b ) Western blot analysis of SORLA levels in scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 bladder cancer cells. GAPDH is a loading control. ( c ) Proliferation of scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 cells. Proliferation data are mean ± s.d. (n = 4 independent experiments; statistical analysis: two-way ANOVA). ( d ) Analysis of tumor growth of subcutaneously injected 5637 cells, with transient SORLA (siSORLA #3) or scramble (siCTRL) silencing, at day 29 in nude mice. Data are displayed as box plots (n = 9-10 mice/group; statistical analysis: unpaired Student’s t -test). ( e ) <t>Ki-67</t> and TUNEL staining of tumor samples prepared as described in (d) and quantifications displayed as box plots (n = 9-10 mice/group; statistical analysis: Mann-Whitney test). ( f ) Schematic illustrating the role of SORLA in regulating the oncogenic fitness of HER2-driven cancer cells. SORLA associates with HER2 via its extracellular domain, co-traffics with HER2 and facilitates HER2 recycling to the plasma membrane to support HER2 downstream signaling. In the absence of SORLA, HER2 becomes localized to enlarged, partially dysfunctional lysosomes resulting in defective HER2 signaling and increased sensitivity to CADs such as ebastine. End: endosome, Lys: lysosome. Box plots represent median and 25 th and 75 th percentiles (interquartile range; IQR) and whiskers extend to maximum and minimum values.
Primary Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibodies/product/Cell Signaling Technology Inc
Average 99 stars, based on 1 article reviews
primary antibodies - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
beta actin  (Proteintech)
93
Proteintech beta actin
SORLA expression correlates with HER2 and promotes tumorigenesis in bladder carcinoma. ( a ) Immunohistological staining of TMA material and correlation analyses of SORLA and HER2 in clinical bladder cancer specimens. Numbers indicate staining intensity, 0=negative, 1=weak, 2=moderate, 3=high. ( b ) Western blot analysis of SORLA levels in scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 bladder cancer cells. GAPDH is a loading control. ( c ) Proliferation of scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 cells. Proliferation data are mean ± s.d. (n = 4 independent experiments; statistical analysis: two-way ANOVA). ( d ) Analysis of tumor growth of subcutaneously injected 5637 cells, with transient SORLA (siSORLA #3) or scramble (siCTRL) silencing, at day 29 in nude mice. Data are displayed as box plots (n = 9-10 mice/group; statistical analysis: unpaired Student’s t -test). ( e ) <t>Ki-67</t> and TUNEL staining of tumor samples prepared as described in (d) and quantifications displayed as box plots (n = 9-10 mice/group; statistical analysis: Mann-Whitney test). ( f ) Schematic illustrating the role of SORLA in regulating the oncogenic fitness of HER2-driven cancer cells. SORLA associates with HER2 via its extracellular domain, co-traffics with HER2 and facilitates HER2 recycling to the plasma membrane to support HER2 downstream signaling. In the absence of SORLA, HER2 becomes localized to enlarged, partially dysfunctional lysosomes resulting in defective HER2 signaling and increased sensitivity to CADs such as ebastine. End: endosome, Lys: lysosome. Box plots represent median and 25 th and 75 th percentiles (interquartile range; IQR) and whiskers extend to maximum and minimum values.
Beta Actin, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/beta actin/product/Proteintech
Average 93 stars, based on 1 article reviews
beta actin - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
industrial sonicator uip2000hdt  (Hielscher Ultrasonics)
90
Hielscher Ultrasonics industrial sonicator uip2000hdt
SORLA expression correlates with HER2 and promotes tumorigenesis in bladder carcinoma. ( a ) Immunohistological staining of TMA material and correlation analyses of SORLA and HER2 in clinical bladder cancer specimens. Numbers indicate staining intensity, 0=negative, 1=weak, 2=moderate, 3=high. ( b ) Western blot analysis of SORLA levels in scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 bladder cancer cells. GAPDH is a loading control. ( c ) Proliferation of scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 cells. Proliferation data are mean ± s.d. (n = 4 independent experiments; statistical analysis: two-way ANOVA). ( d ) Analysis of tumor growth of subcutaneously injected 5637 cells, with transient SORLA (siSORLA #3) or scramble (siCTRL) silencing, at day 29 in nude mice. Data are displayed as box plots (n = 9-10 mice/group; statistical analysis: unpaired Student’s t -test). ( e ) <t>Ki-67</t> and TUNEL staining of tumor samples prepared as described in (d) and quantifications displayed as box plots (n = 9-10 mice/group; statistical analysis: Mann-Whitney test). ( f ) Schematic illustrating the role of SORLA in regulating the oncogenic fitness of HER2-driven cancer cells. SORLA associates with HER2 via its extracellular domain, co-traffics with HER2 and facilitates HER2 recycling to the plasma membrane to support HER2 downstream signaling. In the absence of SORLA, HER2 becomes localized to enlarged, partially dysfunctional lysosomes resulting in defective HER2 signaling and increased sensitivity to CADs such as ebastine. End: endosome, Lys: lysosome. Box plots represent median and 25 th and 75 th percentiles (interquartile range; IQR) and whiskers extend to maximum and minimum values.
Industrial Sonicator Uip2000hdt, supplied by Hielscher Ultrasonics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/industrial sonicator uip2000hdt/product/Hielscher Ultrasonics
Average 90 stars, based on 1 article reviews
industrial sonicator uip2000hdt - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
lipofectamine rnai max  (Thermo Fisher)
90
Thermo Fisher lipofectamine rnai max
SORLA expression correlates with HER2 and promotes tumorigenesis in bladder carcinoma. ( a ) Immunohistological staining of TMA material and correlation analyses of SORLA and HER2 in clinical bladder cancer specimens. Numbers indicate staining intensity, 0=negative, 1=weak, 2=moderate, 3=high. ( b ) Western blot analysis of SORLA levels in scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 bladder cancer cells. GAPDH is a loading control. ( c ) Proliferation of scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 cells. Proliferation data are mean ± s.d. (n = 4 independent experiments; statistical analysis: two-way ANOVA). ( d ) Analysis of tumor growth of subcutaneously injected 5637 cells, with transient SORLA (siSORLA #3) or scramble (siCTRL) silencing, at day 29 in nude mice. Data are displayed as box plots (n = 9-10 mice/group; statistical analysis: unpaired Student’s t -test). ( e ) <t>Ki-67</t> and TUNEL staining of tumor samples prepared as described in (d) and quantifications displayed as box plots (n = 9-10 mice/group; statistical analysis: Mann-Whitney test). ( f ) Schematic illustrating the role of SORLA in regulating the oncogenic fitness of HER2-driven cancer cells. SORLA associates with HER2 via its extracellular domain, co-traffics with HER2 and facilitates HER2 recycling to the plasma membrane to support HER2 downstream signaling. In the absence of SORLA, HER2 becomes localized to enlarged, partially dysfunctional lysosomes resulting in defective HER2 signaling and increased sensitivity to CADs such as ebastine. End: endosome, Lys: lysosome. Box plots represent median and 25 th and 75 th percentiles (interquartile range; IQR) and whiskers extend to maximum and minimum values.
Lipofectamine Rnai Max, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lipofectamine rnai max/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
lipofectamine rnai max - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
lipofectamine 2000  (Thermo Fisher)
90
Thermo Fisher lipofectamine 2000
SORLA expression correlates with HER2 and promotes tumorigenesis in bladder carcinoma. ( a ) Immunohistological staining of TMA material and correlation analyses of SORLA and HER2 in clinical bladder cancer specimens. Numbers indicate staining intensity, 0=negative, 1=weak, 2=moderate, 3=high. ( b ) Western blot analysis of SORLA levels in scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 bladder cancer cells. GAPDH is a loading control. ( c ) Proliferation of scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 cells. Proliferation data are mean ± s.d. (n = 4 independent experiments; statistical analysis: two-way ANOVA). ( d ) Analysis of tumor growth of subcutaneously injected 5637 cells, with transient SORLA (siSORLA #3) or scramble (siCTRL) silencing, at day 29 in nude mice. Data are displayed as box plots (n = 9-10 mice/group; statistical analysis: unpaired Student’s t -test). ( e ) <t>Ki-67</t> and TUNEL staining of tumor samples prepared as described in (d) and quantifications displayed as box plots (n = 9-10 mice/group; statistical analysis: Mann-Whitney test). ( f ) Schematic illustrating the role of SORLA in regulating the oncogenic fitness of HER2-driven cancer cells. SORLA associates with HER2 via its extracellular domain, co-traffics with HER2 and facilitates HER2 recycling to the plasma membrane to support HER2 downstream signaling. In the absence of SORLA, HER2 becomes localized to enlarged, partially dysfunctional lysosomes resulting in defective HER2 signaling and increased sensitivity to CADs such as ebastine. End: endosome, Lys: lysosome. Box plots represent median and 25 th and 75 th percentiles (interquartile range; IQR) and whiskers extend to maximum and minimum values.
Lipofectamine 2000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lipofectamine 2000/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
lipofectamine 2000 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
lipofectamine 3000 reagent  (Thermo Fisher)
90
Thermo Fisher lipofectamine 3000 reagent
SORLA expression correlates with HER2 and promotes tumorigenesis in bladder carcinoma. ( a ) Immunohistological staining of TMA material and correlation analyses of SORLA and HER2 in clinical bladder cancer specimens. Numbers indicate staining intensity, 0=negative, 1=weak, 2=moderate, 3=high. ( b ) Western blot analysis of SORLA levels in scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 bladder cancer cells. GAPDH is a loading control. ( c ) Proliferation of scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 cells. Proliferation data are mean ± s.d. (n = 4 independent experiments; statistical analysis: two-way ANOVA). ( d ) Analysis of tumor growth of subcutaneously injected 5637 cells, with transient SORLA (siSORLA #3) or scramble (siCTRL) silencing, at day 29 in nude mice. Data are displayed as box plots (n = 9-10 mice/group; statistical analysis: unpaired Student’s t -test). ( e ) <t>Ki-67</t> and TUNEL staining of tumor samples prepared as described in (d) and quantifications displayed as box plots (n = 9-10 mice/group; statistical analysis: Mann-Whitney test). ( f ) Schematic illustrating the role of SORLA in regulating the oncogenic fitness of HER2-driven cancer cells. SORLA associates with HER2 via its extracellular domain, co-traffics with HER2 and facilitates HER2 recycling to the plasma membrane to support HER2 downstream signaling. In the absence of SORLA, HER2 becomes localized to enlarged, partially dysfunctional lysosomes resulting in defective HER2 signaling and increased sensitivity to CADs such as ebastine. End: endosome, Lys: lysosome. Box plots represent median and 25 th and 75 th percentiles (interquartile range; IQR) and whiskers extend to maximum and minimum values.
Lipofectamine 3000 Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lipofectamine 3000 reagent/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
lipofectamine 3000 reagent - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
glm procedure  (SAS institute)
90
SAS institute glm procedure
SORLA expression correlates with HER2 and promotes tumorigenesis in bladder carcinoma. ( a ) Immunohistological staining of TMA material and correlation analyses of SORLA and HER2 in clinical bladder cancer specimens. Numbers indicate staining intensity, 0=negative, 1=weak, 2=moderate, 3=high. ( b ) Western blot analysis of SORLA levels in scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 bladder cancer cells. GAPDH is a loading control. ( c ) Proliferation of scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 cells. Proliferation data are mean ± s.d. (n = 4 independent experiments; statistical analysis: two-way ANOVA). ( d ) Analysis of tumor growth of subcutaneously injected 5637 cells, with transient SORLA (siSORLA #3) or scramble (siCTRL) silencing, at day 29 in nude mice. Data are displayed as box plots (n = 9-10 mice/group; statistical analysis: unpaired Student’s t -test). ( e ) <t>Ki-67</t> and TUNEL staining of tumor samples prepared as described in (d) and quantifications displayed as box plots (n = 9-10 mice/group; statistical analysis: Mann-Whitney test). ( f ) Schematic illustrating the role of SORLA in regulating the oncogenic fitness of HER2-driven cancer cells. SORLA associates with HER2 via its extracellular domain, co-traffics with HER2 and facilitates HER2 recycling to the plasma membrane to support HER2 downstream signaling. In the absence of SORLA, HER2 becomes localized to enlarged, partially dysfunctional lysosomes resulting in defective HER2 signaling and increased sensitivity to CADs such as ebastine. End: endosome, Lys: lysosome. Box plots represent median and 25 th and 75 th percentiles (interquartile range; IQR) and whiskers extend to maximum and minimum values.
Glm Procedure, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glm procedure/product/SAS institute
Average 90 stars, based on 1 article reviews
glm procedure - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


SORLA expression correlates with HER2 and promotes tumorigenesis in bladder carcinoma. ( a ) Immunohistological staining of TMA material and correlation analyses of SORLA and HER2 in clinical bladder cancer specimens. Numbers indicate staining intensity, 0=negative, 1=weak, 2=moderate, 3=high. ( b ) Western blot analysis of SORLA levels in scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 bladder cancer cells. GAPDH is a loading control. ( c ) Proliferation of scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 cells. Proliferation data are mean ± s.d. (n = 4 independent experiments; statistical analysis: two-way ANOVA). ( d ) Analysis of tumor growth of subcutaneously injected 5637 cells, with transient SORLA (siSORLA #3) or scramble (siCTRL) silencing, at day 29 in nude mice. Data are displayed as box plots (n = 9-10 mice/group; statistical analysis: unpaired Student’s t -test). ( e ) Ki-67 and TUNEL staining of tumor samples prepared as described in (d) and quantifications displayed as box plots (n = 9-10 mice/group; statistical analysis: Mann-Whitney test). ( f ) Schematic illustrating the role of SORLA in regulating the oncogenic fitness of HER2-driven cancer cells. SORLA associates with HER2 via its extracellular domain, co-traffics with HER2 and facilitates HER2 recycling to the plasma membrane to support HER2 downstream signaling. In the absence of SORLA, HER2 becomes localized to enlarged, partially dysfunctional lysosomes resulting in defective HER2 signaling and increased sensitivity to CADs such as ebastine. End: endosome, Lys: lysosome. Box plots represent median and 25 th and 75 th percentiles (interquartile range; IQR) and whiskers extend to maximum and minimum values.

Journal: bioRxiv

Article Title: SORLA-driven endosomal trafficking regulates the oncogenic fitness of HER2

doi: 10.1101/299586

Figure Lengend Snippet: SORLA expression correlates with HER2 and promotes tumorigenesis in bladder carcinoma. ( a ) Immunohistological staining of TMA material and correlation analyses of SORLA and HER2 in clinical bladder cancer specimens. Numbers indicate staining intensity, 0=negative, 1=weak, 2=moderate, 3=high. ( b ) Western blot analysis of SORLA levels in scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 bladder cancer cells. GAPDH is a loading control. ( c ) Proliferation of scramble (siCTRL) and siSORLA (siSORLA#3, siSORLA#4) silenced 5637 cells. Proliferation data are mean ± s.d. (n = 4 independent experiments; statistical analysis: two-way ANOVA). ( d ) Analysis of tumor growth of subcutaneously injected 5637 cells, with transient SORLA (siSORLA #3) or scramble (siCTRL) silencing, at day 29 in nude mice. Data are displayed as box plots (n = 9-10 mice/group; statistical analysis: unpaired Student’s t -test). ( e ) Ki-67 and TUNEL staining of tumor samples prepared as described in (d) and quantifications displayed as box plots (n = 9-10 mice/group; statistical analysis: Mann-Whitney test). ( f ) Schematic illustrating the role of SORLA in regulating the oncogenic fitness of HER2-driven cancer cells. SORLA associates with HER2 via its extracellular domain, co-traffics with HER2 and facilitates HER2 recycling to the plasma membrane to support HER2 downstream signaling. In the absence of SORLA, HER2 becomes localized to enlarged, partially dysfunctional lysosomes resulting in defective HER2 signaling and increased sensitivity to CADs such as ebastine. End: endosome, Lys: lysosome. Box plots represent median and 25 th and 75 th percentiles (interquartile range; IQR) and whiskers extend to maximum and minimum values.

Article Snippet: For Ki-67 labelling, sections were washed with washing buffer (Tris-HCl 0.05 M pH 7.6, 0.05 % Tween20) and Normal Antibody Diluent (NABD; Immunologic, BD09-125) and incubated with a Ki-67 antibody (14-5698, eBioscience, clone SolA15, diluted 1:2000) for 1 h. After washes, samples were incubated in 3 % H 2 O 2 Tris-HCl for 10 min and washed again.

Techniques: Expressing, Staining, Western Blot, Injection, TUNEL Assay, MANN-WHITNEY